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產品名稱：Invitrogen 12033015 ELECTROMAX DH10B T1 CELLS
貨 號：12033015
規 格：25 reactions
分 類：克隆感受態細胞
品 牌：Invitrogen
國產/進口：進 口
儲存溫度：-20°C
特惠價格：致 電
庫存狀態：現 貨
銷售商：上海慧穎生物科技有限公司
慧穎生物專業供應Invitrogen 12033015 ELECTROMAX DH10B T1 CELLS，常備現貨，*。主營Life Invitrogen產品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規化學試劑、培養基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
Invitrogen 12033015 ELECTROMAX DH10B T1 CELLS描述：
ElectroMAX? DH10B? T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 1010 cfu/μg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction.
? Maximizes yield of transformants from limited cloning products
? Permits efficient cloning of methylated DNA
? Resistant to T1 and T5 bacteriophages
? Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
? Delivers high yield plasmid preparations for downstream applications
Phage-Resistant Representative Libraries from a Single Transformation
The tonA mutation in DH10B? confers resistance to bacteriophages T1 and T5 (1), safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX? DH10B? T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes (2). ElectroMAX? DH10B? T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for applications where there DNA is limiting. The strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B? an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.
Genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697galU galK λ- rpsL nupG tonA
Find the Strain and Format That You Need
DH10B? cells are available in both electrocompetent and chemically competent formats. OurElectroMAX DH10B? Cells are also suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection. Our MegaX DH10B? T1R?Electrocomp? Cells have the highest transformation efficiency (>3 x 1010 cfu/μg) and also offer the benefit of T1 and T5 phage resistance.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Invitrogen 12033015 ELECTROMAX DH10B T1 CELLS同類產品主要有:
| 貨號 | 名稱 | 規格 |
| 14750100 | BLOCKIT ALEXA FLUOR RED OLIGO | 20 UM- 250UL |
| K494500 | BLOCK IT U6 ENTRY VECTOR KIT | 20 RXNS |
| 15630080 | HEPES | 10ml |
| NP0060 | NUPAGE MES BIS-TRIS BFR KIT | COMBO |
| NP0321BOX | NuPAGE? Novex 4-12% Bis-Tris Gel 1.0 mm, 10 Well | 10 gels?box |
| 12355036 | PFX50 DNA POL., 500RXN | 500 RXNS |
| A11180 | CLONEMINER CDNA LIB KIT, VER2 | 5 RXN KIT |
| L150201 | GENERACER SUPERSCRPT TA | 5 CDNA- 50 PCR RXNS |
| 11791020 | LR CLONASE II ENZYME MIX | 20 REACTIONS |
| 10928-034 | 含有Platinum? Taq DNA聚合酶的SuperScriptTM II一步法RT-PCR系統 | 25 rxns |
| 10928-042 | 100 rxns | |
| 11904-018 | 用于RT-PCR的SuperScriptTM II*鏈合成系統 | 50 rxns |
| 18080-200 | SuperScriptTM III CellsDirect cDNA合成試劑盒 | 25 rxns |
| 18080-300 | 100 rxns | |
| 18080-400 | SuperScriptTM III*鏈合成SuperMix | 50 rxns |
| 18068-015 | DNase I擴增級 | 100U(1U/ul) |
| 48190-011 | 隨機引物 | 9 units |
| K210005 | PURELINK PLASMID MIDI 50 | 50 REACTIONS |
| S7563 | SYBR? Green I核酸凝膠染料 | 500ul |
| S7567 | 1ml | |
| S7564 | SYBR? Green II RNA凝膠染料 | 500ul |
| S7568 | 1ml | |
| 11811031 | GENETICIN | 5G |
| 10459014 | DMRIE-C REAGENT | 1ml |
| 12309019 | OPTI PRO SFM | 1000ml |
| 11514015 | PLUS REAGENT | 0.85 ML |
| 12651014 | FREESTYLE CHO EXPRESSION MEDIU | 1000ML |
| 12338018 | FREESTYLE 293 EXPRESSION MED | 1000ML |
| 12347019 | 293FECTIN | 1ml |
| 16447100 | FreeStyle MAX | 1 ml |
| R25001 | ZEOCIN | 1g |
| 15224-017 | T4 DNA 連接酶 | 100U(1U/ul) |
| 15224-025 | 500U(1U/ul) | |
| 15224-090 | 4x500U(1U/ul) | |
| 15224-041 | 250U(5U/ul) | |
| 18047-019 | DNase I | 20,000U |
| 18068-015 | DNase 擴增級 | 100U |
| 18021-014 | 核糖核酸酶H | 30U(2U/ul) |
| 18021-071 | 4x30U(2U/ul) | |
| 18162-016 | DNA聚合酶I/DNase I | 250U |
| 18005-017 | T4 DNA 聚合酶 | 50U(5U/ul) |
| 18005-025 | 250U(5U/ul) | |
| 25530-015 | 蛋白酶K | 100mg |
| 25530-031 | 1g | |
| 18374-058 | 5`RACE系統， | 10 rxns |
| 18373-019 | 3`RACE系統， | 20 rxns |
| MPK10096 | Neon? Transfection System 100 μL Kit | |
| K280020 | ZERO BLUNT TOPO PCRCLON KIT | 20 RXN |
| 18012021 | LARGE FRAGMENT | 100 UN |
| 18021014 | RNASE H (E.COLI) | 30 UN |
| 4311816 | AmpliTaq Gold? DNA Polymerase, 1000 units with Gold Buffer and MgCl2 solution | 1000 un |
| 18092015 | CDNA SIZE FRAC COLUMNS | 3 COLUMNS |
| 450512 | BLOCK-IT H1 RNAI VECTOR KIT | 20 RXNS |
| 10511012 | 1 kb DNA Extension Ladder | 100 UG |
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